S100a10基因对小鼠前体脂肪细胞白色和棕色成脂分化的影响

doi:10.11843/j.issn.0366-6964.2019.06.007

Abstract

Abstract: The objective of this study was to investigate the effects of the S100A10 gene, which was identified as a candidate gene regulating Simmental marbling score by genome-wide association study (GWAS), on adipogenic differentiation of mice preadipocytes. In this study, white preadipocytes were isolated from inguinal white adipose tissue of C57BL/6 mice and induced white/brown adipogenic differentiation in vitro. The expression of the S100a10 gene was interfered using siRNA. The differentiation of preadipocytes were detected by Oil Red O staining. Gene and protein expression levels were measured by qRT-PCR, Western blot and immunofluorescence. The results showed that lipid droplets decreased significantly after S100a10 knocking down. And qRT-PCR assay showed that the expression level of white adipocyte-specific genes Pparg, C/ebpa, Fabp4, Glut4, Adiponectin and Leptin were significantly down regulated in white adipogenic differentiation(P<0.01). The expression level of brown adipocyte-specific genes Ucp1, Pgc1a, Fabp4, Elovl3 and Cox7a were significantly decreased in brown adipogenic differentiation(P<0.01). Western blot result showed that the expression of FABP4 and PPARG were significantly decreased in white adipogenic differentiation(P<0.01), the immunofluorescence assay showed that the expression of FABP4 was decreased in white adipogenic differentiation. The protein expression of brown adipogenic differentiation specific genes FABP4 and UCP1 were obviously decreased in brown adipogenic differentiation(P<0.01). The results showed that knocking down S100A10 inhibited the white/brown adipogenic differentiation in mice preadipocytes. This study provides scientific basis for the further research of the effect of S100A10 on intramuscular fat deposition in beef cattle.

CLC Number:

S823.99

REN Ling, HU Xin, XING Yishen, WANG Yahui, LI Junya, ZHANG Lupei. Effects of S100a10 on White and Brown Adipogenic Differentiation of Mice Preadipocytes[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(6): 1171-1178.

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Effects of S100a10 on White and Brown Adipogenic Differentiation of Mice Preadipocytes

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